Thanks for the reply, Mike. The png is in fact fully opaque. This is the link to one of the png's I used that is all green that shows up as purple in chromakey (https://1drv.ms/u/s!AjoGLBAOTKwok4sEsN_oFHpyx8QhCA). If there is a sample png and/or mp4 that works that I can look at, I can compare and see the differences. Alpha: min: 255 (1) max: 255 (1) mean: 255 (1) %identify -verbose leaf_1280_2.png Image: leaf_1280_2.png Format: PNG (Portable Network Graphics) Mime type: image/png Class: DirectClass Geometry: 1280x616+0+0 Resolution: 118.11x118.11 Print size: 10.8374x5.21548 Units: PixelsPerCentimeter Colorspace: sRGB Type: TrueColorAlpha Base type: Undefined Endianess: Undefined Depth: 8-bit Channel depth: Red: 8-bit Green: 8-bit Blue: 8-bit Alpha: 1-bit Channel statistics: Pixels: 788480 Red: min: 0 (0) max: 232 (0.909804) mean: 53.3939 (0.209388) standard deviation: 35.179 (0.137957) kurtosis: -0.222281 skewness: 0.380167 entropy: 0.873176 Green: min: 3 (0.0117647) max: 255 (1) mean: 143.551 (0.562945) standard deviation: 47.2475 (0.185284) kurtosis: -0.708943 skewness: -0.134383 entropy: 0.947618 Blue: min: 0 (0) max: 229 (0.898039) mean: 12.233 (0.0479725) standard deviation: 14.8046 (0.0580573) kurtosis: 12.2167 skewness: 2.40529 entropy: 0.615689 Alpha: min: 255 (1) max: 255 (1) mean: 255 (1) standard deviation: 0 (0) kurtosis: 8.192e+51 skewness: 1e+36 entropy: 0 Image statistics: Overall: min: 0 (0) max: 255 (1) mean: 116.044 (0.455076) standard deviation: 24.3078 (0.0953246) kurtosis: -1.46953 skewness: 0.326922 entropy: 0.609121 Rendering intent: Perceptual Gamma: 0.45455 Chromaticity: red primary: (0.64,0.33) green primary: (0.3,0.6) blue primary: (0.15,0.06) white point: (0.3127,0.329) Matte color: grey74 Background color: white Border color: srgb(223,223,223) Transparent color: none Interlace: None Intensity: Undefined Compose: Over Page geometry: 1280x616+0+0 Dispose: Undefined Iterations: 0 Compression: Zip Orientation: Undefined Properties: date:create: 2019-03-18T18:29:52-05:00 date:modify: 2019-03-18T18:29:52-05:00 png:bKGD: chunk was found (see Background color, above) png:cHRM: chunk was found (see Chromaticity, above) png:gAMA: gamma=0.45455 (See Gamma, above) png:IHDR.bit-depth-orig: 8 png:IHDR.bit_depth: 8 png:IHDR.color-type-orig: 6 png:IHDR.color_type: 6 (RGBA) png:IHDR.interlace_method: 0 (Not interlaced) png:IHDR.width,height: 1280, 616 png:pHYs: x_res=11811, y_res=11811, units=1 png:sRGB: intent=0 (Perceptual Intent) png:text: 2 tEXt/zTXt/iTXt chunks were found png:tIME: 2019-03-18T13:27:15Z signature: c461e759eb2ea41bf96aa66d74a9c3a21e5f2a23c5d0003e56de23bbdde8eddb Artifacts: verbose: true Tainted: False Filesize: 1.84901MiB Number pixels: 788480 Pixels per second: 15.7696MP User time: 0.070u Elapsed time: 0:01.049 Version: ImageMagick 7.0.8-33 Q16 x86_64 2019-03-13 https://imagemagick.org